Journal: Science China Materials

Article Title: A versatile Pt-Ce6 nanoplatform as catalase nanozyme and NIR-II photothermal agent for enhanced PDT/PTT tumor therapy

doi: 10.1007/s40843-020-1431-5

Figure Lengend Snippet: Figure 10 Changes in weight (a) and tumor volume (b) in mice treated with different methods. Digital pictures of tumors (c) and mice (d) at the 14th day after treatment. TUNEL staining (e) and H&E staining (f) of tumor tissues at the 14th day. The nuclei and apoptotic cells were stained blue (DAPI staining) and red (TUNEL staining). (g) A representative immunofluorescent image of tumor slices stained with HIF-1α. The p values in (a) were calculated by Tukey’s posttest (

Article Snippet: The following antibodies were used in Western blot and immunofluorescence staining: Bax, Bcl-2, Hsp90, Cyt-c, HIF-1α and COX-IV (Proteintech), cleaved Caspase-3 (Sigma), as well as GAPDH (Goodhere Biotech).

Techniques: TUNEL Assay, Staining

Journal: bioRxiv

Article Title: TAT-Cx43 266-283 impairs metabolic plasticity in glioma stem cells in vitro and in vivo

doi: 10.1101/858563

Figure Lengend Snippet: ( a ) GSCs were recorded by time-lapse microscopy in medium containing only glucose or only amino acids (Videos 1 and 2, respectively). After 4 days, these media were changed to complete medium and cells were recorded for a further 24 h (nutrient replenishment). Note that cells that appeared dead (bright, round shape) could survive and proliferate after nutrient replenishment, which was not true for cells maintained in medium with no nutrients at all (Supplementary Fig. 3 and Video 3). Scale bar: 100 μm. ( b–e ) GSCs were treated with 50 μM TAT or TAT-Cx43 266-283 in complete medium for 24 h. Then, the medium was replaced with medium containing glucose (glucose-only medium), amino acids (amino acid-only medium) or both glucose and amino acids, as well as 50 μM of each peptide, for another 24 h. For comparison, cells were treated in parallel with complete medium and 50 μM of peptides. Then, the indicated assays were carried out. ( b ) MTT oxidation of GSCs under the indicated conditions. ( c ) GSCs were recorded by time-lapse microscopy in complete or glucose-only medium (Videos 4–9; the images are the last photomicrograph from each video) or photographed under the indicated conditions. Scale bar: 100 μm. ( d ) Representative western blots of the indicated proteins under the indicated conditions. GSCs treated with TAT-Cx43 266-283 in glucose-only medium exhibit decreased levels of HIF-1α, GLUT-3, HK-2, G6PD and Sox-2, but not HK-1 (right panel). However, GSCs treated in complete medium showed only slight decreases (left panel). GAPDH and α-tubulin blots are shown as loading controls. All data are mean ± s.e.m. and were obtained from at least three independent experiments with at least two technical replicates (one-way ANOVA: *** P < 0.001 vs control; ### P < 0.001 vs TAT; + P < 0.05, ++ P < 0.01, +++ P < 0.001 between the indicated conditions). Numbers under bars indicate the number of biological replicates in ( b ).

Article Snippet: After blocking, the membranes were incubated overnight at 4 °C with primary antibodies: mouse monoclonal antibody against α-actinin (1:1,000; Millipore, MAB1682), mouse monoclonal antibody against G6PD (1:250; Santa Cruz Biotechnology, sc-373886), mouse monoclonal antibody against glyceraldehyde phosphate dehydrogenase (GAPDH; 1:5.000; Invitrogen, AM4300), rabbit polyclonal antibody against GLUT-1 (1:500–1:1.000; Millipore, 07-1401), mouse monoclonal antibody against GLUT-3 (1:100; Santa Cruz Biotechnology, sc-74497), rabbit polyclonal antibody against HIF-1α (1:200; Novus Biologicals, NB100-479), rabbit monoclonal antibody against HK-1 (1:250; Cell Signaling Technology, 2024), mouse monoclonal antibody against HK-2 (1:500; Thermo Fisher, MA5-15679), mouse monoclonal antibody against RPL-19 (1:200; Santa Cruz Biotechnology, sc-100830), rabbit polyclonal antibody against Sox-2 (1:500; Abcam, ab97959), Total OXPHOS Rodent WB Antibody Cocktail against NDUFB8 (complex I), SDHB (complex II), UQCRC2 (complex III), MTCO1 (complex IV) and ATP5A (complex V) (1:250; Abcam, ab110413), and mouse monoclonal antibody against α-tubulin (1:1,000; Sigma-Aldrich, T9026).

Techniques: Time-lapse Microscopy, Comparison, Western Blot

Journal: American journal of physiology. Regulatory, integrative and comparative physiology

Article Title: PDH activation during in vitro muscle contractions in PDH kinase 2 knockout mice: effect of PDH kinase 1 compensation.

doi: 10.1152/ajpregu.00498.2010

Figure Lengend Snippet: Fig. 2. PDK activity in WT and PDK2KO hindlimb muscle mitochondria. Values are means SE (n 5). Insets: Western blots of protein from WT and PDK2KO mice. A: PDK activity in intact mitochondria isloated from mixed hindlimb muscles, reported as first-order rate constant for ATP-dependent inhibition of PDHa. B: PDK2 content. AU, arbitrary units. C: PDK4 content. D: PDK1 content. *Significantly different from WT (P 0.05).

Article Snippet: Membranes were washed with TBST and incubated for 1 h in 5% milk-TBST containing secondary antibodies: for PDK2, PDK4, and HIF-1 , a 1:20,000 dilution of goat anti-rabbit horseradish peroxidase (HRP)-conjugated IgG (Santa Cruz Biotechnology, Santa Cruz, CA); for PDK1, bovine anti-goat HRP-conjugated IgG (Santa Cruz Biotechnology); and for COX IV, E1 , and E2, anti-mouse HRP (26).

Techniques: Activity Assay, Western Blot, Muscles, Inhibition

Journal: American journal of physiology. Regulatory, integrative and comparative physiology

Article Title: PDH activation during in vitro muscle contractions in PDH kinase 2 knockout mice: effect of PDH kinase 1 compensation.

doi: 10.1152/ajpregu.00498.2010

Figure Lengend Snippet: Fig. 2. PDK activity in WT and PDK2KO hindlimb muscle mitochondria. Values are means SE (n 5). Insets: Western blots of protein from WT and PDK2KO mice. A: PDK activity in intact mitochondria isloated from mixed hindlimb muscles, reported as first-order rate constant for ATP-dependent inhibition of PDHa. B: PDK2 content. AU, arbitrary units. C: PDK4 content. D: PDK1 content. *Significantly different from WT (P 0.05).

Article Snippet: Membranes were washed with TBST and incubated for 1 h in 5% milk-TBST containing secondary antibodies: for PDK2, PDK4, and HIF-1 , a 1:20,000 dilution of goat anti-rabbit horseradish peroxidase (HRP)-conjugated IgG (Santa Cruz Biotechnology, Santa Cruz, CA); for PDK1, bovine anti-goat HRP-conjugated IgG (Santa Cruz Biotechnology); and for COX IV, E1 , and E2, anti-mouse HRP (26).

Techniques: Activity Assay, Western Blot, Muscles, Inhibition

Journal: American journal of physiology. Regulatory, integrative and comparative physiology

Article Title: PDH activation during in vitro muscle contractions in PDH kinase 2 knockout mice: effect of PDH kinase 1 compensation.

doi: 10.1152/ajpregu.00498.2010

Figure Lengend Snippet: Fig. 2. PDK activity in WT and PDK2KO hindlimb muscle mitochondria. Values are means SE (n 5). Insets: Western blots of protein from WT and PDK2KO mice. A: PDK activity in intact mitochondria isloated from mixed hindlimb muscles, reported as first-order rate constant for ATP-dependent inhibition of PDHa. B: PDK2 content. AU, arbitrary units. C: PDK4 content. D: PDK1 content. *Significantly different from WT (P 0.05).

Article Snippet: Membranes were washed with TBST and incubated for 1 h in 5% milk-TBST containing secondary antibodies: for PDK2, PDK4, and HIF-1 , a 1:20,000 dilution of goat anti-rabbit horseradish peroxidase (HRP)-conjugated IgG (Santa Cruz Biotechnology, Santa Cruz, CA); for PDK1, bovine anti-goat HRP-conjugated IgG (Santa Cruz Biotechnology); and for COX IV, E1 , and E2, anti-mouse HRP (26).

Techniques: Activity Assay, Western Blot, Muscles, Inhibition